Discrimination of Modes of Action of Antifungal Substances by Use of Metabolic Footprinting

Created by W.Langdon from gp-bibliography.bib Revision:1.4192

  author =       "Jess Allen and Hazel M. Davey and David Broadhurst and 
                 Jem J. Rowland and Stephen G. Oliver and 
                 Douglas B. Kell",
  title =        "Discrimination of Modes of Action of Antifungal
                 Substances by Use of Metabolic Footprinting",
  journal =      "Applied and Environmental Microbiology",
  year =         "2004",
  volume =       "70",
  number =       "10",
  pages =        "6157--6165",
  month =        oct,
  keywords =     "genetic algorithms, genetic programming",
  DOI =          "doi:10.1128/AEM.70.10.6157-6165.2004",
  abstract =     "Diploid cells of Saccharomyces cerevisiae were grown
                 under controlled conditions with a Bioscreen
                 instrument, which permitted the essentially continuous
                 registration of their growth via optical density
                 measurements. Some cultures were exposed to
                 concentrations of a number of antifungal substances
                 with different targets or modes of action (sterol
                 biosynthesis, respiratory chain, amino acid synthesis,
                 and the uncoupler). Culture supernatants were taken and
                 analyzed for their metabolic footprints by using
                 direct-injection mass spectrometry. Discriminant
                 function analysis and hierarchical cluster analysis
                 allowed these antifungal compounds to be distinguished
                 and classified according to their modes of action.
                 Genetic programming, a rule-evolving machine learning
                 strategy, allowed respiratory inhibitors to be
                 discriminated from others by using just two masses.
                 Metabolic footprinting thus represents a rapid,
                 convenient, and information-rich method for classifying
                 the modes of action of antifungal substances.",
  notes =        "PMID:",

Genetic Programming entries for Jess Allen Hazel M Davey David I Broadhurst Jem J Rowland Stephen G Oliver Douglas B Kell