Differentiation of Phytophthora infestans Sporangia from Other Airborne Biological Particles by Flow Cytometry

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@Article{day:2002:AEM,
  author =       "Jennifer P. Day and Douglas B. Kell and 
                 Gareth W. Griffith",
  title =        "Differentiation of Phytophthora infestans Sporangia
                 from Other Airborne Biological Particles by Flow
                 Cytometry",
  journal =      "Applied and Environmental Microbiology",
  year =         "2002",
  volume =       "68",
  number =       "1",
  pages =        "37--45",
  month =        jan,
  keywords =     "genetic algorithms, genetic programming",
  URL =          "http://intl-aem.asm.org/cgi/reprint/68/1/37.pdf",
  DOI =          "doi:10.1128/AEM.68.1.37-45.2002",
  abstract =     "The ability of two different flow cytometers, the
                 Microcyte (Optoflow) and the PAS-III (Partec), to
                 differentiate sporangia of the late-blight pathogen
                 Phytophthora infestans from other potential airborne
                 particles was compared. With the PAS-III, light scatter
                 and intrinsic fluorescence parameters could be used to
                 differentiate sporangia from conidia of Alternaria or
                 Botrytis spp., rust urediniospores, and pollen of
                 grasses and plantain. Differentiation between P.
                 infestans sporangia and powdery mildew conidia was not
                 possible by these two methods but, when combined with
                 analytical rules evolved by genetic programming
                 methods, could be achieved after staining with the
                 fluorescent brightener Calcofluor white M2R. The
                 potential application of these techniques to the
                 prediction of late-blight epiphytotics in the field is
                 discussed.",
  notes =        "GMax-Bio",
}

Genetic Programming entries for Jennifer P Day Douglas B Kell Gareth W Griffith

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