School of Computer Science

Imaging Laboratory

Professor Ela Claridge, Dr Iain Styles

SpectraCube Hyperspectral Imaging System

The Multispectral Imaging Laboratory supports research in the Medical Image Analysis Group and is part of the University's Collaborative Research Network in Imaging and Visualisation.

The laboratory is equipped with state-of-the-art multispectral and hyperspectral imaging devices and accessories, including:

  • SpectraCube (Applied Spectral Imaging) - a hyperspectral imaging system which uses a Sagnac interferometer to record "interferograms" at each pixel which are then Fourier-transformed to give spectral information. Images can be acquired in the visible range (300 - 900 nm). Post-processing software has extensive facilities for interactive analysis of images and spectra and for linear unmixing and classification.
  • VariSpec VIS filter (C.R.I) - electronically controlled liquid crystal filter capable of acquiring images with 1 nm resolution in the visible range from 400 to 700 nm. The filter can be attached to a monochrome camera to acquire a sequence of narrow-band images from which a spectral cube ("lambda stack") is built.
  • VariSpec NIR filter (C.R.I) - as above, but working in the near-infrared range, from 650 to 1200 nm.
  • Nuance (C.R.I) - a multispectral imaging system comprising VariSpec filter and a scientific camera for acquisition and processing of microscopy images, both bright field and fluorescence. The system includes software for de-illumination, linear unmixing and classification, both supervised (using spectral libraries) and unsupervised (automatic).
  • High-grade high sensitivity scientific cameras, capable of imaging in visible and near-infrared range.
  • Integrating sphere (ProLite) with calibrated halogen light source and bespoke facilities for providing uniform illumination through different apertures.
  • Spectralon calibration tiles.
  • A range of lenses, from macro to telephoto, filters and other accessories.
Image Analysis Software
Hyperspectral Image Analysis Software

All the equipment is calibrated and therefore can be used for quantitative work.

The laboratory is housed in a dark room which ensures that experiments can be carried out under controlled light conditions.

In addition to commercially provided software there is a range of software facilities developed in-house, from controlling the imaging devices and filters, through calibration, to sophisticated quantitative image interpretation.

The group have extensize experience using the imaging devices in a variety of application domains, including ophthalmology, fluorescence microscopy and geology, and the equipment can be connected to most optical imaging systems.